Mammalian orthoreovirus can exit cells in extracellular vesicles.

"The authors have declared that no competing interests exist."

"Research reported in this work was supported by the National Institutes of Health (R01AI155646 to KMO; 1F31AI167541 to SCS), the National Center for Advancing Translational Sciences (CTSA Award no. UL1 TR002243), and by Dolly Parton Pediatric Infectious Diseases Research Funds. SCS was supported by the Chemical Biology of Infectious Diseases Training Program (NIH T32AI112541). Services at the Vanderbilt Cell Imaging Shared Resource performed through Vanderbilt University Medical Center’s Digestive Disease Research Center were supported by National Institutes of Health grant P30DK058404 Core Scholarship. The Cell Imaging Shared Resource and EK were also supported by NIH CA68485, DK20593, DK58404, DK59637 and EY08126. The contents of this publication are solely the responsibility of the authors and do not necessarily represent the views of the National Institutes of Health or the National Center for Advancing Translational Sciences. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript."