The role of <i>SLC12A</i> family of cation-chloride cotransporters and drug discovery methodologies.

"other disadvantages include size and potential effects on cell integrity are discussed in section s8 in the supplementary data 3 11 to investigate the intracellular chloride concentration one method is to utilize a tracer uptake assay using 36 cl - to characterize cl - conductance involving measuring the intracellular accumulation of radioactive chloride."

"Declaration of competing interest Except for Dong Liang, who is an employee of Aurora Discovery Inc., all authors declare that there are no conflicts of interest."

"We are very grateful for the financial support from the 10.13039/501100001809National Natural Science Foundation of China (Grant Nos.: 82170406, 81970238, and 32111530119), Shanghai Municipal Science and Technology Major Project, China (Grant No.: 2018SHZDZX01), The 10.13039/501100000288Royal Society UK (Grant No.: IEC\NSFC\201094), and the 10.13039/501100000867Commonwealth Scholarship Commission UK (Grant No.: NGCA-2020-43). Shiyao Zhang: and Nur Farah Meor Azlan: Writing - Original draft preparation, Reviewing and Editing; Sunday Solomon Josiah and Jing Zhou: Writing - Reviewing and Editing; Xiaoxia Zhou and Lingjun Jie: Resources, Investigation; Yanhui Zhang: Investigation, Supervision; Cuilian Dai: Supervision; Dong Liang: Resources, Investigation; Peifeng Li and Zhengqiu Li: Investigation, Supervision; Zhen Wang: Resources, Investigation; Yun Wang: Supervision, Funding acquisition; Ke Ding: Conceptualization, Supervision; Yan Wang: Supervision, Project administration, Funding acquisition; Jinwei Zhang: Project administration, Supervision, Conceptualization, Validation, Writing - Original draft preparation, Reviewing and Editing, Resources, Funding acquisition."