Periapical bacterial disinfection is critical for dental pulp regenerative cell therapy in apical periodontitis in dogs.

"Declarations Ethics approval and consent to participateAll animal procedures were approved by the Animal Care and Use Committee of the National Center for Geriatrics and Gerontology, Research Institute (Title of Experiment: Development of dentin and pulp regeneration with dental pulp stem cells for caries, pulpitis, and root canal treatment, permission #2-17 (Jan 15th 2020), #3-47 (Jan 16th, 2021), #4-43 (Jul 7th, 2023)) and Aichi Medical University (Title of Experiment: Development of novel caries, pulpitis, and root canal therapy by dentin and pulp regeneration with tissue stem cells, permission #2020-93 (Aug 7th, 2020), #2021-85 (Jun 24th, 2021), #2022-57 (Aug 29th, 2022)). Consent for publicationNot applicable. Competing interestsThe author Nakashima M has financial relationship with Air Water Inc. No other author has reported a potential competing interests relevant to this article. Competing interests The author Nakashima M has financial relationship with Air Water Inc. No other author has reported a potential competing interests relevant to this article."

"Funding This work was supported by a Joint Research and Development between National Center for Geriatrics and Gerontology and Air Water Inc. The funding body played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript."