Chemoproteomics Reveals USP5 (Ubiquitin Carboxyl-Terminal Hydrolase 5) as Promising Target of the Marine Polyketide Gracilioether A.

"gel bands were then excised from the gel and submitted to trypsin digestion for the next bottom-up nano-esi-uplc-ms/ms analysis (data available via proteomexchange with identifier pxd048098).; the mass spectrometry proteomics data have been deposited to the proteomexchange consortium via the pride [ ] partner repository with the data set identifier pxd048098.; supplementary materials the following supporting information can be downloaded at: https://www mdpi com/article/10 3390/md22010041/s1 figure s1: list of transitions of usp5 tryptic peptides selected for the analysis of the lip experiment; figure s2: usp5 peptides identified with the lip experiment involved in binding with gea; figure s3: binding mode of hhy (in violet) superimposed to its pose (in green) in the crystal structure of usp5 (pdb code: 6dxt); figure s4: uncropped image displaying the gel related to one of the darts experiments; figure s5: uncropped image of the western blot confirmation of darts experiments; figure s6: darts data for usp5; figure s7: peak area of peptide transition analyzed in the t-lip experiments; figure s8: plot of the ru values obtained through spr analysis in function of gea concentrations [ 16 19 20 21 22 ]."

"Conflicts of Interest The authors declare no conflicts of interest."

"This research was funded by the University of Salerno (FARB 2021) ORSA215145 project, and by Ministero dell’Università e della Ricerca (MUR)—PRIN2022 project, “2022RCTY7M, BiTMaP: Coupling the power of bioinformatics and experimental tools for accelerating the production, structural elucidation, and target identification of marine natural products” and PRIN2022-PNRR project, “P2022CKMPW, TACSI Driver: a multitasks platform to guide the Target identification, Assessment of the binding, Collection of natural products from waste, Synthesis of derivatives, and In vitro/In vivo polypharmacological profile evaluation of bioactive compounds”."