Epitranscriptional m6A modification of rRNA negatively impacts translation and host colonization in Staphylococcus aureus.

"plos publication stage vor-update-to-uncorrected-proof publication update 2024-02-01 data availability the ribo-seq and mrna-seq data reported in this paper have been deposited in the ncbi gene expression omnibus (geo) database with accession number gse168265.; data availability the ribo-seq and mrna-seq data reported in this paper have been deposited in the ncbi gene expression omnibus (geo) database with accession number gse168265.; processed and raw sequencing data were deposited in the ncbi geo database with accession number gse168265. we found that a large fraction of genes involved in metabolism and information processing and many well-characterized virulence genes were concordantly downregulated at the transcript and translation levels including many that are required for cell adhesion such as fnbab the sdr operon nuc2 sasg and spa ( fig 3b-3d and s1 dataset ).; g/ml) were determined by e-test on muller hinton agar plates in duplicates per strain per antibiotic type (pdf) click here for additional data file s2 table (pdf) click here for additional data file s3 table (pdf) click here for additional data file s4 table (pdf) click here for additional data file s1 dataset (xlsx) click here for additional data file s1 fig (a-c) s aureus strains (je2 ( ermbl -- ermb - ) ermbl r7stop - ermb wt ermbl r7stop - ermb y103a ermbl r7stop - ermb i75t/n100s ) were grown overnight in tsb at 37? file s4 table (pdf) click here for additional data file s1 dataset (xlsx data availability the ribo-seq and mrna-seq data reported in this paper have been deposited in the ncbi gene expression omnibus (geo) database with accession number gse168265"

"The authors have declared that no competing interests exist."

"This study was supported by the National Institutes of Health grants R01AI150986 (to MNFY) and R01GM121359 (to MNFY) and Department of Defense W81XWH-18-1-0122 (to MNFY), and in part by the PEW Charitable Trusts Grant #2920 (to MNFY) and the Edward Mallinckrodt Jr. Foundation (to MNFY). TEM analysis was performed at the Northwestern University Center for Advanced Microscopy generously supported by NCI CCSG P30 CA060553. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript."