Immunoseroproteomic profiling in autoantibody to ENO1 as potential biomarker in immunodiagnosis of osteosarcoma by serological proteome analysis (SERPA) approach.

"the immunostaining pattern was compared with available experimental gene/protein characterization data in the uniprotkb/swiss-prot database and other experimental evidence for location described in scientific literature for consistency u2-os and saos-2 were cultured and lysed in rehydration buffer (cat# 1632106 bio-rad hercules ca)."

"Disclosure statement The authors report no conflict of interest."

"Author contributions: J.Y.Z. and W.Y.L. designed the study and obtained funding. J.T.L. drafted the manuscript. J.T.L. and L.P.D. performed the experiments. J.T.L. and Y.M. did the statistical analysis and prepared all figures. M.Y.H. collected the samples and images. J.T.L. contributed to the acquisition and interpretation of the data. Z.P.G. and X.W. critically reviewed and revised the article for important intellectual content. All authors approved the final manuscript and decided to submit the article for publication."