ST2-Mediated Neutrophilic Airway Inflammation: A Therapeutic Target for Patients With Uncontrolled Asthma.

"immune cells (neutrophils and monocytes) were isolated using isolation kits and purified by using their expressed cd markers as shown in supplementary data s1 and supplementary fig s1 .; nets were induced and isolated from asthmatic patients ( supplementary data s1 ).; finally these antibodies had an effect on inhibiting inflammatory cell infiltration and mucus production in the lungs ( fig 6e ) suggesting st2 serves as a novel target for managing neutrophilic airway inflammation in na ( supplementary data s2 supplementary fig s3 s8 s9 s10 s11 ).; supplementary materials supplementary data s1 materials and methods supplementary data s2 results supplementary table s1 key materials supplementary table s2 isolation kits and recombinant proteins supplementary table s3 enzyme-linked immunosorbent assay kits supplementary table s4 antibodies for western blot and immunofluorescence supplementary table s5 antibodies for flow cytometry supplementary table s6 the used machines and software in this study supplementary table s7 the sequences of the primers supplementary fig s1 purification of human neutrophils and classical monocytes from asthmatic patients (a) gating strategy to identify neutrophils (cd45 + cd16 + cd66b + ) before and after magnetic isolation (b) gating strategy to identify classical monocytes (cd45 + cd14 + cd16 - ) before and after magnetic isolation."

"Disclosure: There are no financial or other issues that might lead to conflict of interest."

"This study was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2020R1I1A1A01073900) and a grant from the Korean Health Technology R & D Project, Ministry of Health & Welfare, Republic of Korea (HR16C0001)."