Diacylglycerols and Lysophosphatidic Acid, Enriched on Lipoprotein(a), Contribute to Monocyte Inflammation.

"this rna-seq from the ex vivo monocyte experiments data has been made publicly available at the ncbi (national center for biotechnology information) gene expression omnibus and is accessible through the geo series accession number gse249911. the r scripts are available on zenodo and can be accessed at doi org/10 5281/zenodo 8435690. all lipidomics readouts have been made available in data sets s1 through s3 .; figures s1-s9 major resources table tables s1-s4 data sets s1-s3 full unedited blots for figure 6 r script s1 nonstandard abbreviations and acronyms atx autotaxin casp1 caspase 1 cvd cardiovascular disease dg diacylglycerol ec endothelial cell haec human arterial endothelial cell hdl high-density lipoprotein hrp horseradish peroxidase il interleukin ldl low-density lipoprotein llp1 lipid phosphate phosphatase 1 lp-pla2 lipoprotein-associated phospholipase a2 lp(a) lipoprotein(a) lpa lysophosphatidic acid lpc lysophosphatidylcholine lpl lysophospholipase mgat monoacylglycerol acyltransferase nf-?"

"the r scripts are available on zenodo and can be accessed at doi org/10 5281/zenodo 8435690.; r scripts are available on github and can be accessed at https://github com/atheroexpress/microanatomyhumanplaque_scrnaseq . data are visualized in tsne (t-distributed stochastic neighbor embedding) plots and violin plots using the r script in the supplemental material (code: scrnaseq plots).; the r code is available in the supplemental material (pipeline lipidomics analysis); (2) identification of metabolites using an in-house database of phospholipids with known internal standards indicating the position of most of the lipid clusters matching m/z values within 3-ppm deviation; (3) isotope correction to obtain deconvoluted intensities for overlapping peak groups; (4) normalization on the intensity of the internal standard for lipid classes for which an internal standard was available and scaling on measured protein content per sample; (5) total value is the sum of the relative abundance of all identified lipid species; (6) a student t test with post hoc bonferroni correction was used for statistical comparison between the 2 groups."

"Disclosures S. Tsimikas is a coinventor and receives royalties from patents owned by the University of California San Diego (UCSD), is a cofounder and has an equity interest in Oxitope Pharma and Kleanthi Diagnostics, and has a dual appointment at UCSD and Ionis Pharmaceuticals. The other authors report no conflicts."

"Sources of Funding This work was supported by the Netherlands Organization for Scientific Research. J. Kroon received a VENI grant from ZonMW (91619098) and was supported by the Dutch Heart Foundation (Senior Scientist Dekker grant 03-004-2021-T045). This work was funded by the European Union (European Research Council (ERC) starting grant, ENDOMET-STEER, 101076407). The views and opinions expressed are, however, those of the authors only and do not necessarily reflect those of the European Union or the European Research Council Executive Agency. Neither the European Union nor the granting authority can be held responsible for them. J. Kroon received a preclinical research grant from Oxitope Pharma B.V. S. Tsimikas was supported by National Institutes of Health (NIH) R01 HL159156. The Pelacarsen studies were funded by Ionis Pharmaceuticals."