Mononuclear phagocyte system-related multi-omics features yield head and neck squamous cell carcinoma subtypes with distinct overall survival, drug, and immunotherapy responses.

"fig 1 the flowchart of the present study table 1 the summary characteristics of the included samples in this study datasets tcga-hnsc sources data types samples tcga gene expression rnaseq 481 range mean age 20-90 61 54 no % age 19-39 16 3 33 40-49 51 10 60 50-59 115 23 91 60-88 299 62 16 gender female 131 27 23 male 350 72 77 clinical stage stage i 18 3 74 stage ii 93 19 34 stage iii 102 21 20 stage iva 253 52 60 stage ivb 9 1 87 stage ivc 6 1 25 radiation therapy yes245 50 94 no 132 27 44 not reported 104 21 62 gse65858 sources data types samples geo gene expression rnaseq 270 range mean age 35-87 60 12 no % age 19-39 2 0 74 40-49 39 14 44 50-59 100 37 04 60-88 129 47 78 gender female 47 17 40 male 223 82 60 clinical stage stage i 18 6 67 stage ii 37 13 70 stage iii 37 13 70 stage iva 155 57 41 stage ivb 16 5 93 stage ivc 7 2 59 treatment uni-modality 78 28 89 multi-modality 189 70 00 palliative 3 1 11 gse41613 sources data types samples geo gene expression rnaseq 97 no % age 19-39 6 6 19 40-49 16 16 49 50-59 28 28 87 60-88 47 48 45 gender female 31 31 96 male 66 68 04 clinical stage stage i/ii 41 42 27 stage iii/iv 56 57 73 treatment uni-modality 43 44 33 multi-modality 53 54 64 not reported 1 1 03 gse195832 sources data types samples geo single-cell rnaseq not applicable through diminishing the batch effort four raw scrna-seq samples presented a uniform and random distribution under the umap reduction (figure s1 a) 25 cell clusters were gathered of which a smaller number responded to more cells (figure s1 b)."

"the classifier now has been uploaded to github ( https://github com/cqmuzc/getmpsub ).; notably for the convenience of future research we have packaged the classifier using the ntp algorithm into an r package called "getmpsub" and uploaded it to github for easy accessibility.; to make our study reproducible and truly applicable to clinical research we uploaded our packaged classifier to github."

"Declarations Conflict of interestThe authors declare that they have no competing interests. Ethics approval and consent to participateNot applicable. Consent for publicationNot applicable. Conflict of interest The authors declare that they have no competing interests."

"Funding This research was funded by (National Youth Science Foundation Project), grant number [82204159]) and by (Postdoctoral Fund project of Chongqing), grant number (cstc2021jcyj-bshX0220)."