Regulation of YAP Promotor Accessibility in Endothelial Mechanotransduction.

"sequencing data derived from this study are deposited in the gene expression omnibus under the given accession code (geo accession: gse253761).; analysis of ranked less-accessible peaks indicated yap as a transcriptional start site with reduced accessibility in huvecs (log 2 [fold change] -1 1596; p =0 0174; data s1 and s2 [geo accession: gse253761]).; figures s1-s11 tables s1 and s2 data s1 and s2 (geo accession: gse253761) major resources table nonstandard abbreviations and acronyms aaa abdominal aortic aneurysm amotl2 angiomotin-like 2 atac-seq assay for transposase-accessible chromatin sequencing chip-seq chromatin immunoprecipitation sequencing ec endothelial cell ecm extracellular matrix edu 5-ethynyl-2?-deoxyuridine fak focal adhesion kinase fss fluid shear stress huaec human umbilical arterial endothelial cell huvec human umbilical vein endothelial cell hrp horseradish peroxidase ivc inferior vena cava linc linker of nucleoskeleton and cytoskeleton prc polycomb repressive complex pla proximity ligation assay pol ii rna polymerase ii wt wild type yap yes-associated protein for sources of funding and disclosures see page 687. analysis of ranked less-accessible peaks indicated yap as a transcriptional start site with reduced accessibility in huvecs (log 2 [fold change] -1 1596; p =0 0174; data s1 and s2 [geo accession: gse253761]).; results of peak accessibility regardless of significance can be found in data s1 (geo accession: gse253761).; figures s1-s11 tables s1 and s2 data s1 and s2 (geo accession: gse253761) major resources table nonstandard abbreviations and acronyms aaa abdominal aortic aneurysm amotl2 angiomotin-like 2 atac-seq assay for transposase-accessible chromatin sequencing chip-seq chromatin immunoprecipitation sequencing ec endothelial cell ecm extracellular matrix edu 5-ethynyl-2?-deoxyuridine fak focal adhesion kinase fss fluid shear stress huaec human umbilical arterial endothelial cell huvec human umbilical vein endothelial cell hrp horseradish peroxidase ivc inferior vena cava linc linker of nucleoskeleton and cytoskeleton prc polycomb repressive complex pla proximity ligation assay pol ii rna polymerase ii wt wild type yap yes-associated protein for sources of funding and disclosures see page 687."

"Disclosures None."

"Sources of Funding This study was supported by grants from the Swedish Heart and Lung Foundation (L. Holmgren and J. Roy), Novo Nordisk Foundation (NNF15CC0018346; L. Holmgren), Swedish Cancer Society (L. Holmgren), Swedish Childhood Cancer Foundation (L. Holmgren), Cancer Society of Stockholm (L. Holmgren), Swedish Research Council (L. Holmgren), Knut and Alice Wallenberg Foundation (L. Holmgren), European Research Council under the EU Horizon 2020 research and innovation programme (grant agreement 773076; P. Saharinen), Sigrid Jusélius Foundation (P. Saharinen), Cancer Foundation Finland (P. Saharinen), Academy of Finland (P. Saharinen)."