Liter-scale manufacturing of shelf-stable plasmid DNA/PEI transfection particles for viral vector production.

"Declaration of interests The authors declare the following competing financial interests: Y.H., Y.Z., and H.-Q.M. are inventors of patent applications on the NP assembly method described in this paper, filed through, and managed by Johns Hopkins Technology Ventures. B.A.E. and T.G. are employees of 2seventy bio, Inc., and hold company stock."

"This work was supported by 2seventy bio, Inc. and 10.13039/100000054National Cancer Institute (NCI) through a grant (R01CA281143) to H.-Q.M. The authors would like to thank Nolan D. Sutherland for input on the overall study design; David R. Wilson and Prof. Jordan J. Green from the Department of Biomedical Engineering at Johns Hopkins University for establishing the B16F10-Gal8-mRuby3 cell line; Stipe Iveljic and Rich Middlestadt from the 10.13039/100014582Johns Hopkins University, Whiting School of Engineering Machine Shop for manufacturing of the CIJ devices; Yicheng Zhang from the Department of Materials Science and Engineering at Johns Hopkins University for assistance in formulation experiments; Sarvani Manne, Chiu-Ping Liu, Lucille Altman-Sagan, and David Kotlikoff from the vector process development team at 2seventy bio, Inc. for supporting LVV production experiments; and the analytical team at 2seventy bio, Inc. for characterization of the yield and quality of the LVVs produced in this study."