Advanced patient-specific microglia cell models for pre-clinical studies in Alzheimer's disease.

"single-cell rna sequencing data have been deposited in the ncbi sequence read archive (sra) with bioproject accession number prjna1067365 o ( https://www ncbi nlm nih gov/sra/prjna1067365 ). a comprehensive list of significantly differentially expressed genes ( p < 0 05) in each individual cluster can be found in additional file 2 : dataset s1.; correlation of the pseudo-bulk expression profile of a subset of 798 core microglial genes [ ] confirmed that 3d co-culture mdmi are more similar to olah et al (r = 0 70) and svoboda et al (r = 0 57) than the other culture systems (additional file 1 : fig s8; additional file 3 : dataset s2).; all differentially expressed genes ( p < 0 05) within each individual cluster for every model system are listed in additional file 4 : dataset s3.; (ifn-?) compared to hc (fig 3 g-i; additional file 5 : dataset s4).; exposure also elicited disease-specific inflammatory responses in the 3d co-cultures with il-6 being significantly secreted at lower levels in ad compared to hc (fig 3 p; additional file 5 : dataset s4).; dataset s1.; dataset s2.; dataset s3.; dataset s4. additional file 5. dataset s4. raw data"

"codes have been deposited in https://github com/ankit277/scrna ."

"Declarations Ethics approval and consent to participateAll research adhered to the ethical guidelines on human research outlined by the National Health and Medical Research Council of Australia (NHMRC) and ethical approval was obtained from QIMRB-MRI. All participants provided informed consent before participating in the study. Consent for publicationNot applicable. Competing interestsA.R.W. and H.Q. are listed as co-authors of provisional patent AU2020/050513. The authors declare no other competing interests. Competing interests A.R.W. and H.Q. are listed as co-authors of provisional patent AU2020/050513. The authors declare no other competing interests."

"Funding This research was funded by the National Health and Medical Research Council of Australia (NHMRC) (APP1125796), and a National Foundation for Medical Research and Innovation (NFMRI) Grant to A.R.W. The PISA project is funded by the National Health and Medical Research Council (NHMRC) (APP1095227). CC-L was recipient of The University of Queensland PhD scholarship. MKL was supported by an NHMRC Boosting Dementia Leadership Fellowship (APP1140441). ARW was supported by an NHMRC Senior Research Fellowship (APP1118452). HQ was supported by a 10X Millennium Science Fellowship."