Application of gold immunochromatographic assay strip combined with digital evaluation for early detection of Toxoplasma gondii infection in multiple species.

"availability of data and materials the gene sequences of ama1 of toxoplasma gondii generated and/or analyzed during this study are available in ncbi repository genbank: xm_002364813 1. availability of data and materials the gene sequences of ama1 of toxoplasma gondii generated and/or analyzed during this study are available in ncbi repository genbank: xm_002364813"

"Declarations Ethics approval and consent to participateThe study was reviewed and approved by permission from the Ethics Committee of the Second People’s Hospital of Lishui, China (Approval File No. 20220705-1). Four female New Zealand rabbits (2–2.5 kg) were kept in the experimental animal house at Center of Zhejiang Academy of Medical Sciences, Zhejiang Province, China, and all steps of operation for animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of Hangzhou Medical College (Zhejiang Academy of Medical Sciences) (Approval File No. 2021-010). Additionally, all rabbits were allowed for experimentation by the breeders. Samples from cats and dogs were approved by the Hangzhou Medical College Institutional Animal Care and Use Committee (Approval File No: 2023-038) and followed the Chinese legislation about the use and care of research animals (GB/T35823-2018). All methods were performed in accordance with the relevant guidelines and regulations and conducted as per the Animal Research: Reporting in Vivo Experiments (ARRIVE) Guidelines. Consent for publicationNot applicable. Competing interestsThe authors declare that they have no competing interests. Competing interests The authors declare that they have no competing interests."

"Funding This work was supported by the National Natural Science Foundation of China (No. 81871684 & No. 32370997), Provincial Key R & D program of Zhejiang Department of Science and Technology (2019C03057), Health Commission of Zhejiang Province (2024KY923), Chinese Medicine Science and Technology Program of Zhejiang Province (2024ZL367), Zhejiang Medical and Health Science and Technology Plan (grant No. WKJ-ZJ-2203), Zhejiang Province “Pioneer” and “Leading Goose” R&D plan (grant No. 2022C03109), and Central Leading Local Science and Technology Development Fund Project (grant No. 2023ZY1019)."