The bloodstream form of Trypanosoma brucei displays non-canonical gluconeogenesis.

"plos publication stage vor-update-to-uncorrected-proof publication update 2024-03-06 data availability the data is available from the figshare database https://figshare com/authors/julie_kovarova/17814896 .; data availability the data is available from the figshare database https://figshare com/authors/julie_kovarova/17814896 .; the raw data is publically available from the figshare depository ( https://figshare com/authors/julie_kovarova/17814896 ) 2 x 10 7 cells were used per sample. s1 table (xlsx data availability the data is available from the figshare"

"The authors have declared that no competing interests exist."

"This work was supported by CZ.02.2.69/0.0/0.0/19_074/0016248 LeishWeb to J.K.; by the Ministry of Education, Youth and Sports of the Czech Republic grant RNA for therapy (CZ.02.01.01/00/22_008/0004575), by the European Research Council (ERC, MitoSignal, grant agreement no. 101044951), and GACR 20-14409S to A.Z.; D. H. was funded by an Investigator Award from Wellcome [217105/Z/19/Z]. M.P.B was funded by an MRC Newton grant ‘Bridging epigenetics, metabolism and cell cycle in pathogenic trypanosomatids’ MR/S019650/1. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript."