Meta-Transcriptomic Analysis Uncovers the Presence of Four Novel Viruses and Multiple Known Virus Genera in a Single <i>Hibiscus rosa-sinensis</i> Plant in Colombia.

"in contrast hicav-d and genbank-deposited carlavirus sequences shared only 31 43% and 40 40% amino acid identity with 91% and 66% query coverage (148 aa) with spc6v (yp_006589923 1) and hennv (acm45995 1) respectively ( figure 5 b).; the hicav-a hicav-b hicav-c and hicav-d genome sequences were deposited in genbank under accession numbers pp115947 pp115948 pp115949 and pp115956-59 respectively.; the blastn analysis of hivx tgb1 and tgb3 did not show any significant nucleotide identity with available virus sequences in genbank but tgb2 shared 73 68-77 94% nt identity with 17-24% genome coverage with the lily symptomless carlavirus (lc720794 1 and om311155 1).; the hivx cpg and tgb3 protein shared 52 61-54 50% and 38 18-47 37% nt identities with 66-69% and 85-90% query coverage respectively with the four closest virus sequences [yucca alphaflexivirus1 (qqg34624 1) phyvx (uep18565 1) agave potexvirus1 (qqg34617 1) and yvx (yp_009091818 1)] available in genbank.; the hivx genome sequence was deposited in genbank under accession number pp115950.; the phyvnv-hib rna1 and rna2 genome sequences were deposited in genbank under accession numbers pp115951 and pp115952 respectively.; the hcrsv-rsthr isolate genome sequence was deposited in genbank under accession number pp115955.; after bridging the gap of rna2 segment both the pfgsv-rna1 and -rna2 genome sequences were deposited in genbank under accession numbers pp115953 and pp115954 respectively.; thus the carlavirus isolate thl detected in this sample is presumed to be a strain of hicav-b and the genome sequence was deposited in genbank under accession number pp115960.; data availability statement the data presented in the study are deposited in the ncbi repository at https://www ncbi nlm nih gov/ under accession numbers pp115947-pp115960 and bioproject id prjna1063669. data availability statement the data presented in the study are deposited in the ncbi repository at https://www ncbi nlm nih gov/ under accession numbers pp115947"

"Conflicts of Interest The authors declare no conflicts of interest, as the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest."

"This research was funded by USDA-ARS in-House Appropriated project NP303-8042-22000-319-000-D and USDA-APHIS PPQ Citrus Health Response Program (CHRP) Project No. PPQ-013258."