Immune response after oral immunization of goats and foxes with an NDV vectored rabies vaccine candidate.

"the 1 6 kb pcr product was gel-purified using the qiaquick gel extraction kit (qiagen hilden germany) and was subsequently inserted into a cloning vector pucndvh5 [ ] by phusion polymerase chain reaction (finnzymes phusion new england biolabs) [ ] thereby replacing the h5 insert from pucndvh5 with the g orf resulting in pucndv_g rabv in which the g orf is inserted between ndv f and hn genes flanked by ndv hn ncr ( s1 data ).; beyond rabies it is likely that ndv based vaccines generally elicit an immune response after oral delivery thus giving hope to vaccines for animal diseases where parenteral vaccinations face limitations particularly in the outreach to remote communities in lmics s1 data double underlined sequences present the ndv f gene end sequence at the 5' end or the ndv hn gene start sequence at the 3' end respectively."

"The authors have declared that no competing interests exist."

"The author(s) received no specific funding for this work."