Potential selection of antimony and methotrexate cross-resistance in Leishmania infantum circulating strains.

"plos publication stage vor-update-to-uncorrected-proof publication update 2024-03-12 data availability the mass spectrometry proteomics data have been made accessible through the supplementary file s1 data .; data availability the mass spectrometry proteomics data have been made accessible through the supplementary file s1 data .; the complete proteomics dataset is available in ( s1 data ).; we obtained and analyzed quantitative data to determine the thermal stability of 1147 soluble proteins ( s1 data ).; among the 1193 soluble proteins identified ( s1 data ) 42 exhibited a pattern that allowed us to calculate melting curves ( fig 5a and 5b ).; among the 1112 soluble proteins identified ( s1 data ) melting curves were determined for 55 of them.; differences were statistically evaluated using an unpaired two-tailed t-test ** p<0 01; *** p<0 001 (tif) s1 data (xlsx) authors want to thank dr aida minguez-menendez for her help with the conception of the figures."

"The authors have declared that no competing interests exist."

"Work in the CFP-Lab was supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant (RGPIN-2017-04480 to CFP) and by the Canada foundation for Innovation (grant no. 37324 and 38858 to CFP). The proteomic investigations were funded by the NSERC (RGPIN-2020-05228 to FB), and proteomics laboratory equipment was funded by the Canadian Foundation for Innovation (grant no. 36706 to FB). FB holds the Canada Research Chair in metrology of bioactive molecule and target discovery (CRC-2021-00160 to FB). This work was supported by the Instituto de Salud Carlos III through the ISCIII-AESI (project PI21CIII/00005 to JM). JCS was supported by CIBERINFEC (CB21/13/00018 to JM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript."