<i>Brucella abortus</i> triggers the differential expression of immunomodulatory lncRNAs in infected murine macrophages.

"these were identified by performing bioinformatic analysis on publicly available raw sequencing data (geo accession: gse100914) derived from total rna samples extracted from bone marrow macrophages (bmm) infected with brucella abortus strain 544. we then identified those transcripts present in both infected and uninfected raw264 7 cells to calculate the log2 infected:uninfected ratio and defined it as log2 (fold change tpm) ( supplementary data 2 ).; public raw rna-seq data sets (geo accession: gse100914) were processed and analyzed to determine the transcriptional landscape of lncrnas including lethe lincrna-cox2 lincrna-eps malat1 and gas5 ( supplementary data 1 ).; public raw rna-seq data sets ( https://www ncbi nlm nih gov/sra/prjna694452 ) were processed and analyzed to obtain the expression profile of these specific immunomodulatory lncrnas ( supplementary data 2 ).; when analyzing interaction networks of these 49 genes with genes related to the immune response we found a total of 73 interactions suggesting that these lncrnas could exert regulatory roles of the immune response mainly at the nuclear level ( supplementary data 3 ).; the analysis revealed that 165 lncrnas were differentially expressed in bmm in response to b abortus infection ( supplementary data 1 ) similar to the 130 differentially expressed lncrnas previously reported using raw264 7 cells ( ).; some interaction networks formed by genes such as ifnb1 or cxcl2 stand out for showing a large number of associated interactions ( supplementary data 3 ) suggesting that the modulation of the expression of both genes are affected by changes in the expression of these lncrnas during b abortus infection."

"Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest."

"The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by FONDECYT grant 1230018 awarded to AO."