Molecular mapping of neuronal architecture using STORM microscopy and new fluorescent probes for SMLM imaging.

"the use of fluorescent labeling in conjunction with 3d microscopy led to the production of large amounts of published data that are on the way to being classified and accessible through various infrastructures or free repositories (ebrains zenodo etc).; code and data availability tutorial and download concerning soda plugin and user-friendly "easy soda protocol" can be found on icy software website here: https://icy bioimageanalysis org/protocol/easy-soda-2-colors-1-image/ sample image is also available here on zenodo: https://zenodo org/record/4323312- y_3tqx1cdhq a french tutorial on ripley's function and icy soda plugin can be found on youtube: https://www youtube com/watch?"

"Disclosures: The authors have no competing interests to declare and are not financially interested or remunerated for MemBright probe sales."

"We would like to thank the NeurImag Core Facility team (part of IPNP, Inserm U. 1266 and Université Paris Cité) and member of the National Infrastructure France-BioImaging supported by the French National Research Agency (ANR-10-INBS-04) for their technical and scientific support. We thank the Leducq Foundation for supporting the acquisition of the Leica SP8 Confocal/STED 3DX microscope and DIM Elicit and FRC/Rotary for funding the Bruker Vutara STORM and Optera spinning disc systems, respectively. VB, DB, and PN were supported by the FLAG-ERA (Grant Senseï by ANR-19-HBPR-0003), ANR-19-CE16-0012-03, and ANR-22-CE17-010-02. This work was supported by grants from the Agence Nationale de la Recherche (ANR-19-CE16-0012-03; ANR-22-CE17-0010-02; ANR-21-CE11-0010-03) and FLAG-ERA Grant Senseï by ANR-19-HBPR-0003 to LD."