Microbiome changes through the ontogeny of the marine sponge Crambe crambe.

"a specific sequence similarity search using the basic local alignment search tool (blast) available on the national center for biotechnology information (ncbi) platform was performed for the most dominant asv (asv00001) to assess whether or not it belonged to the already described dominant symbiont for c crambe.; availability of data and materials raw sequence data are available from the ncbi sra under project prjna990364 ( https://www ncbi nlm nih gov/sra/? availability of data and materials raw sequence data are available from the ncbi sra under project prjna990364"

"Declarations Ethics approval and consent to participateThe study is exempt from ethics approval since, according to article 3.1 of the European Union directive (2010/63/UE) from 22/9/2010, no approval is needed for sponge scarification, as they are the most primitive animals and lack a nervous system. Moreover, the collected sponges are not listed in any list of endangered species. Consent for publicationNot applicable. Competing interestsThe authors declare that they have no competing interests. Competing interests The authors declare that they have no competing interests."

"Funding Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This study was primarily supported by two grants from the Spanish Ministry of Science and Innovation (PID2019-105769GB-I00 and RYC2018-024247-I) and an internal grant from CSIC (PIE-202030E006) to AR. Additionally, CDV received the support of a fellowship from the “la Caixa” Foundation (ID 100010434)”, code LCF/BQ/PI22/11910040. Finally, part of this research was supported through the SponBIODIV project (AR and MM), a 2021–2022 BiodivProtect joint call for research proposals, under the Biodiversa + Partnership co-funded by the European Commission and with the funding organizations ‘Fundación Biodiversidad’ and FORMAS. MT was supported by a JdC (Juan de la Cierva Formación, 2020) personal grant (FJC2020-043677-I)."