Using microarrays to facilitate positional cloning: identification of tomosyn as an inhibitor of neurosecretion.

"we hybridized total rna isolated from wild-type and kp3365 unc-43(n1186) camkii mutant animals to the affymetrix c elegans genechip ( dataset s1 ).; we then hybridized rna from kp3293 nu468 animals to the c elegans genechip comparing the hybridizations to wild-type hybridizations as previously described ( dataset s1 ).; dataset s1 (2 mb tds) click here for additional data file."

"Competing interests. The authors have declared that no competing interests exist."

"We thank K. Vranizan for statistical and programming advice; T. Rector for Affymetrix sample preparation and hybridizations; J. Rine, D. Altshuler, V. Mootha, S. McCarroll, and C. Murphy for helpful discussions; T. Speed for statistical input; P. Juo, L. Drier, J. Dittman, and I. Ruvinsky for reading this manuscript; other members of the Kaplan and Ruvkun labs for suggestions; K. Scott for providing a fantastic foster lab for MD in Berkeley; and Gregoire for sustenance. Some of the strains described here were provided by the C. elegans Genetic Stock Center. This work was supported by a grant from the National Institutes of Health to JK (GM54728). MD was supported by a Howard Hughes Medical Institute predoctoral fellowship."