Monitoring SARS-CoV-2 variants in wastewater of Dhaka City, Bangladesh: approach to complement public health surveillance systems.

"red colored circular strains are from this study table 2 detection of sars-cov-2 variants in wastewater surveillance first reported in human first outbreak first human case* first report in ww* variant who label transmissibility wastewater sample type may 2020 south africa january 2020 april 2021 b 1 351 beta 50% sewage treatment facilities september 2020 uk february 2020 january 2021 b 1 1 7 alpha 43-90% sewage october 2020 india b 1 617 1 kappa - wwtp october 2020 india march 2020 september 2020 b 1 617 2 delta - wwtp [ ] november 2020 usa b 1 526 iota 20% wwtp november 2020 brazil april 2020 may 2021 p 1 gamma 152% wwtp november 2021 botswana november 2021 november 2021 b 1 1 529 omicron - wastewater (florida) may 2021 (bangladesh) - - - b 1 617 2 delta - - this study (in wastewater sample) - - - b 1 b 1 1 b 1 1 25 b 1 617 2 delta - septic tanks drains surface water pumping station ww wastewater *first human case is based on the sequence deposited on gisaid database table 3 mutation analysis in sequences detected in our wastewater surveillance study sample id gisaid accession number genome size (bp) amino acid mutations030106 epi_isl_17398164 29847 nsp2:i120f; nsp3:a1766v p1228l p77l; nsp4:a446v; nsp12:p323l; spike:d614g; n:r203k g204r 0101244 epi_isl_17398165 29851 nsp2:i120f; nsp3:a1941t; nsp4:a446v nsp12:p323l; nsp15:d128e; nsp16:t117i; spike:d614g; t676i; ns3:w193r; n:r203k g204r 0101082 epi_isl_17398166 29839 nsp2:i120f w450c; nsp3:s1296f; nsp12:p323l nsp13:e261d; spike:d614g q677h t719i; n:r203k g204r 0101142 epi_isl_17398167 29878 nsp2:i120f; ns3:t223i; nsp6:v149f; nsp12:p323l; nsp15:t33i m:a142v; spike:d614g; n:r203k g204r 030105 epi_isl_17398168 29865 nsp2:p129l; nsp3:p822l; nsp4:a446v; nsp12:p323l spike:d614g; m:i82t; n:r203k g204r 0101309 epi_isl_17398169 29878 nsp2:p129l; ns3:s26l; nsp4:a446v; nsp12:p323l; spike:d614g p681r; n:r203k g204r 0401132 epi_isl_17398170 29838 nsp3:m1547i; nsp4:a446v; nsp5:p99l; nsp6:t77a; nsp12:p323l g671s; spike:d614g; n:r203m 202080 epi_isl_17398171 29771 nsp3:p1228l a488s v1234l p1469s; nsp4:v167l t492i; nsp6:a76v t77a; ns7b:t40i; nsp8:p183l nsp12:p323l g671s; nsp13:p77l; nsp14:a394v spike: t19r e156g r158del f157del g142d d614g p681r; m:i82t; n:g215c r203m d377y 0102275 epi_isl_17398172 29845 nsp1:r24c; nsp2:i120f; spike:t19r a222s d614g p681r a924s; n:r203k g204r 0102089 epi_isl_17398173 29771 nsp3: s26l h682y; nsp4:t492i; nsp6:v149a; nsp12: t51i p323l g671s; nsp13:p77l v558l; nsp15:h234y; spike: t19r e156g g142d f157del r158del d614g p681r; m:i82t; n:g215c r203m sars-cov-2 environmental surveillance in wastewater has several epidemiological implications."

"Declarations Competing interestsThe authors declare no competing interests. Ethics approval and consent to participateNot applicable. Consent for publicationNot applicable. Competing interestThe authors declare that they have no competing interests. Competing interests The authors declare no competing interests. Competing interest The authors declare that they have no competing interests."

"Funding This work was supported by Kurita Water and Environmental Foundation; Grant No. 21P015. Rehnuma Haque is supported by the Global Health Equity Scholars Program NIH FIC and NIEHS D43 TW010540."