Upregulation of WDR6 drives hepatic de novo lipogenesis in insulin resistance in mice.

"data availability all rna-sequencing datasets have been deposited in the gene expression omnibus and are publicly available (fig 1c and supplementary data fig 1 ( gse205460 ) and extended data fig 8 ( gse205459 )).; proteomics and phosphoproteomics data have been deposited at the proteomexchange consortium via the pride partner repository and are publicly available under accession numbers pxd034367 and pxd034542 . the level of phospho-ppp1cb (thr316) was examined in liver samples of wdr6 liver-specific knock-in (wdr6-lki; supplementary data 4 ) and wdr6-lko mice using custom primary antibody that specifically recognizes the phospho-ppp1cb at thr316.; the primers used for genotyping of mice are provided in supplementary data 6 .; primer sequences for adenoviral and adeno-associated viral plasmid construction are listed in supplementary data 7 .; the primer sequences of genotyping are listed in supplementary data 6 .; supplementary information supplementary methods supplementary data figs1-5 and supplementary data 6-10 (tables including primer sequences for genotyping plasmid construction rt-pcr and antibodies).; reporting summary supplementary data 1 statistical source data.; supplementary data 2 unprocessed western blots. data availability all rna-sequencing datasets have been deposited in the gene expression omnibus and are publicly available (fig 1c and supplementary data fig 1 ( gse205460"

"Competing interests The authors declare no competing interests."

"This work was supported by the National Natural Science Foundation of China (91957209 to L.G., 81770788 to T.B., 82130025 to J.Z. and 82200879 to Z.Y.), Taishan Scholar Project special funding (to L.G., T.B. and J.Z.) and the Outstanding University Driven by Talents Program and Academic Promotion Program of Shandong First Medical University (2019LJ007 to J.Z.). We are grateful to Y. Liu (Wuhan University, China) for kindly providing research strategies in lipid metabolism. We are grateful to Y. Guan (East China Normal University) for kindly providing Srebp1c−/−(gene ID: 20787) mice. We thank X. Hou (Shandong University, China) and L. Jiang (Fuzhou University, China) for kindly providing suggestions of molecular docking and MD simulation analysis. We thank the College of Artificial Intelligence and Big Data for Medical Science, Shandong First Medical University, for kindly providing suggestions for clinical data analysis. We also thank L. Zhao, J. Xie, B. Xiang, N. Wang, X. Sun and W. Bian for technical assistance."