A Chemoproteomic Approach to Elucidate the Mechanism of Action of 6-Azasteroids with Unique Activity in Mycobacteria.

"data availability statement proteomics data sets from this study are deposited in the massive database under reference msv000091952."

"The authors declare no competing financial interest."

"The research is funded by NIH R01AI134054 and R01A141526 (subcontract from Colorado State University) to N.S.S. The MIC and cytotoxicity assays are partially supported by the National Institutes of Allergy and Infectious Diseases Preclinical Service Contract 75N93019D00005. The proteomic experiments were conducted at the Stony Brook Proteomics Center. The authors would like to thank Dr. John Haley from the Proteomics Core Facility at Stony Brook University for performing proteomic analysis, including on-bead digestion workup, liquid chromatography of digested peptides, and operation of Proteome Discoverer software to generate protein lists. Shearson Editorial Services (Cornwall, NY, USA) provided English language editing of the text of this paper. Author Contributions: Joshua Werman: Conceptualization, Methodology, Validation, Formal analysis, Investigation, Data curation, Writing—Original Draft, Visualization. Yu-Ching Chen: Methodology, Validation, Formal analysis, Investigation, Data curation, Visualization. Tianao Yuan: Conceptualization, Methodology, Investigation, Writing—Review and Editing. Xinxin Yang: Methodology, Investigation. Nicole Sampson: Conceptualization, Resources, Writing—Review and Editing, Supervision, Funding acquisition."