Measuring cell-to-cell expression variability in single-cell RNA-sequencing data: a comparative analysis and applications to B cell aging.

"these pre-sorted hscs were sequenced with full-length smart-seq2 sequencing protocols which can be downloaded from ncbi gene expression omnibus (geo; http://www ncbi nlm nih gov/geo/ ) under accession number gse100428. codes to reproduce the presented analyses and figures are available at github ( https://github com/huiwenzh/cell-to-cell-variability-changes-in-ageing ) [ 71 ] and have been deposited to zenodo via accession identifier (10 5281/zenodo 8210511) [ 25 ].; we also provide implementations of these 14 metrics compared in this work as scvar github package ( https://github com/huiwenzh/scvar ) [ 17 ] which have been deposited on zenodo via accession identifier (10 5281/zenodo 8166143) [ 54 ]. table 2 . scater [ ] is applied to normalize the raw data"

"codes to reproduce the presented analyses and figures are available at github ( https://github com/huiwenzh/cell-to-cell-variability-changes-in-ageing ) [ 71 ] and have been deposited to zenodo via accession identifier (10 5281/zenodo 8210511) [ 25 ].; we also provide implementations of these 14 metrics compared in this work as scvar github package ( https://github com/huiwenzh/scvar ) [ 17 ] which have been deposited on zenodo via accession identifier (10 5281/zenodo 8166143) [ 54 ]."

"Declarations Ethics approval and consent to participateNot applicable. Consent for publicationNot applicable. Competing interestsThe authors declare that they have no competing interests. Competing interests The authors declare that they have no competing interests."

"Funding Australian Research Council Future Fellowship (FT170100047) and a Georgina Sweet Award to J.C.M. A Next Step Initiative Grant from the Australian Academy of Technology and Engineering to H.Z. and J.C.M."