Tracking arboviruses, their transmission vectors and potential hosts by nanopore sequencing of mosquitoes.

"finally this method can form the basis of an early warning detection system by identifying arboviruses before spillovers into human populations providing a system for pre-empting future arboviral epidemics fastq files for amplicon and metagenomic sequences generated using nanopore sequencing for these experiments have been deposited and made publicly available in the european nucleotide archive (ena) under the project accession number prjeb67345 study accession id erp152382 fastq files are available at ena under accession numbers erx11591481-erx11591610."

"s w wrote co1_fish and the python script for database management and uploaded the fastq files to e n a l d o g v h j t and k k collected and identified mosquitoes provided for this project."

"Conflicts of interest N.L. and J.Q. have received grant funding from Oxford Nanopore Technologies. All authors declare that there are no additional conflicts of interest."

"Funding information This research benefited from the State Research Institutes Modernization Program, supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (2017/50345-5); Medical Research Council-São Paulo Research Foundation (FAPESP) CADDE partnership award (MRC MR/S0195/1 and FAPESP 18/14389–0) (https://caddecentre.org). L.d.O.G. was supported by a postdoctoral fellowship (FAPESP 2018/16232-1). K.K. is a CNPq research fellow (309396/2021–2). I.M.C was supported by the Bill and Melinda Gates Foundation (INV-034540). N.J.L. and J.Q. are supported by the Wellcome ARTIC network (206298)."