Integrative genomic analyses reveal putative cell type-specific targets of the Drosophila ets transcription factor Pointed.

"data availability all raw data are uploaded onto gene expression omnibus accession number: gse247258. among the top 20 motifs enriched in individual anti-flag and anti-gfp chip-seq peaks ten motifs are common to at least four out of the five replicates (supplementary data 1 ).; the remaining top 20 motifs enriched in individual chip-seq peaks are shown in supplementary data 1 .; as expected the ets motif (bound by pnt and anterior open/yan) is among the top three motifs identified in anti-gfp and anti-flag chip-seq peaks of all replicates (supplementary data 1 ).; as expected some of the top enriched go terms are associated with processes related to eye function and development (supplementary data 2 ).; we intersected single cell genomics datasets with anti-gfp and anti-flag chip-seq datasets; this yielded 157 or 145 genes in the anti-gfp or anti-flag chip-seq datasets respectively that are specifically expressed in r1-7 or cone cells (supplementary data 3 - 5 ).; motif analyses using peaks that map to these 145 genes from anti-gfp and anti-flag repeats identified six factors that appear in at least four out of the five replicates: ets croi aef1 klu lame duck (lmd) and buttonhead (btd) (supplementary data 6 ).; supplementary data 6 shows a complete list of factors (with e-values) identified in the individual repeat analyses.; in addition 47 anti-gfp and 46 anti-flag genes are expressed in a cell type-specific manner in late larval eye discs (supplementary data 5 ).; other putative novel cell type-specific targets of pnt including factor of interpulse interval ( fipi ) (supplementary fig 6 a-c) are shown in supplementary data 5 . data availability all raw data are uploaded onto gene expression omnibus accession number: gse247258"

"Declarations Consent for publicationNot applicable. Ethical approvalNot applicable. Competing interestsGM is co-owner of Genetivision Corporation. Competing interests GM is co-owner of Genetivision Corporation."

"Funding This work was supported in part by the Retina Research Foundation."