A transcriptomic examination of encased rotifer embryos reveals the developmental trajectory leading to long-term dormancy; are they "animal seeds"?

"the datasets generated during the current study are available ncbi accession prjna821380 id 821380 at https://www ncbi nlm nih gov/bioproject/prjna821380 .; in addition the b plicatilis transcriptome and cel-seq data are available at ncbi accession prjna821380 id 821380 at https://www ncbi nlm nih gov/bioproject/prjna821380 . availability of data and material data are available in the main text or the supplementary materials. in addition the b plicatilis transcriptome and cel-seq data are available at ncbi accession prjna821380"

"to identify differences in transcript abundance profiles between ams and res over the course of development the 3'-end cel-seq reads from the single embryo samples were aligned to the reference transcriptome (available at ncbi accession no prjna821380 samples srr21541574-srr21541589 using bowtie2 ( https://bowtie-bio sourceforge net/bowtie2/index shtml v 2 2 6) and relative transcript abundance were estimated using rsem ( https://github com/deweylab/rsem v1 2 25)."

"Declarations Ethics approval and consent to participateThis study involves experimental studies of rotifers, an invertebrate group of organisms. There are no ethical regulations regarding laboratory experimental studies of this group of organisms. Consent for publicationNot applicable. Competing interestsThe authors declare no competing interests. Competing interests The authors declare no competing interests."

"Funding The Israel Science Foundation grant #1524/13 (EL). The Israel Ministry of Agriculture grant #14–16-005 (EL, YGA). The funding bodies were not involved in the design of the study, collection, analysis and interpretation of data or in writing the manuscript."