Insights from Melipona bicolor hybrid genome assembly: a stingless bee genome with chromosome-level scaffold.

"this final genome assembly and its annotation as well as all datasets used to generate them are available at ncbi under the bioproject prjna632864 and accession code jahyiq01.; transcriptomic data used for gene prediction is deposited at ncbi under the sra accession srx5527788 and it consisted of 327 5 million paired reads.; availability of data and materials the datasets generated and/or analyzed during the current study are available in the ncbi repository bioproject prjna632864 and accession code jahyiq01. availability of data and materials the datasets generated and/or analyzed during the current study are available in the ncbi repository bioproject prjna632864"

"the repetitive elements final library and report are available at ( https://github com/nat2bee/repetitive_elements_pipeline/tree/master/database ).; repetitive elements were identified in the genome using the pipeline available at https://github com/nat2bee/repetitive_elements_pipeline .; then we concatenated our custom library with the dfam v3 1 hymenoptera library included in repeatmasker v4 1 0 [ ] and used the same program to annotate and soft mask the repeats found in the genome based on our custom library (available at https://github com/nat2bee/repetitive_elements_pipeline )."

"Declarations Ethics approval and consent to participateNot applicable. Consent for publicationNot applicable. Competing interestsThe authors declare no competing interests. Competing interests The authors declare no competing interests."

"Funding This study was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) grants 2019/23186–9 and 2016/24669–5, and by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, research sponsorship to MCA, Proc. 307356/2019–1). NSA was funded by the Fonds De La Recherche Scientifique (FNRS) grant number 40005980. This manuscript article was published with the financial support of the Fondation Universitaire de Belgique and CNPq."