The genome and transcriptome of the snail Biomphalaria sudanica s.l.: immune gene diversification and highly polymorphic genomic regions in an important African vector of Schistosoma mansoni.

"pacbio ccs reads (raw reads available in ncbi bioproject: prjna1041389) were assembled using flye [ ] settings: flye -pacbio-hifi bsud111_ccs fasta -g 1 g -i 0 -t 16 -o flye/bsud111.; aligned genome data and raw reads are available in archive ncbi sequence read archive (ncbi bioproject: prjna1041389).; esl was funded by nih grant r37ai101438' availability of data and materials the datasets generated and/or analyzed during the current study are available in the ncbi bioproject prjna1041389. availability of data and materials the datasets generated and/or analyzed during the current study are available in the ncbi bioproject prjna1041389"

"the bioinformatic pipeline for the genome annotation is available at github com/j-calvelo/annotation-biomphalaria-sudanica. of the original 6945781 circular consensus sequencing (ccs) reads3798283 (54 68%) passed the q20 quality threshold determined in the longqc software [ ] of which 1708667 (44 99%) were identified as potentially complete isoforms (i e they bear both 5' and 3' adapter sequences) using lima (github com/pacificbiosciences/barcoding) (supplementary table 1 )."

"Declarations Ethics approval and consent to participateThis project was undertaken following approval from the relevant bodies, including Kenya Medical Research Institute (KEMRI) Scientific Review Unit (Approval # KEMRI/RES/7/3/1 and KEMRI/SERU/CGHR/035/3864), Kenya’s National Commission for Science, Technology, and Innovation (License # NACOSTI/P/15/9609/4270 and NACOSTI/P/22/14839), Kenya Wild- life Services (permit # 0004754 and # WRTI-0136–02-22), and National Environment, Management Authority (permit # NEMA/AGR/46/2014 – Registration # 0178 and NEMA/AGR/159/2022 – Registration # 201). Consent for publicationNot applicable. Competing interestsNot applicable. Competing interests Not applicable."

"Funding Funding for this project was provided by National Institutes of Health, National Institute of Allergy and Infectious Disease R01AI141862, which supported TP, JAT, GO, FR, kA, BM, MOdh., MOdi. and MLS. ESL was funded by NIH grant R37AI101438.’"