The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies.

Publication Year: 2024

DOI:
10.1099/mgen.0.001198

PMCID:
PMC10926689

PMID:
38376377

Journal Information

Full Title: Microb Genom

Abbreviation: Microb Genom

Country: Unknown

Publisher: Unknown

Language: N/A

Publication Details

Subject Category: Microbiology

Available in Europe PMC: Yes

Available in PMC: Yes

PDF Available: No

Transparency Score
4/6
66.7% Transparent
Transparency Indicators
Click on green indicators to view evidence text
Core Indicators
Evidence found in paper:

"pmc pmc10926689 pmc10926689 10926689 38376377 38376377 001198 10 1099/mgen 0 001198 research articles genomic methodologies the long and short of it: benchmarking viromics using illumina nanopore and pacbio sequencing technologies http://mgen microbiologyresearch org *correspondence: andrew millard adm39@le ac uk all reads from virome sequencing were submitted to the ena under the study prjeb56639.; data availability - all reads from virome sequencing were submitted to the ena under study prjeb56639.; these findings will provide a starting point for others in the choice of reads and assembly algorithms for the analysis of viromes bacteriophage bioinformatics hybrid assembly illumina long-reads nanopore pacbio phage viral metagenomics viromics openaccessembargo 0 all reads from virome sequencing were submitted to the ena under study prjeb56639. the assemblies are provided via figshare (10 25392/leicester data 21346935) 2024 20 2 2024 20 2 2024 [ comment ] 10 2 001198 25 2 2023 25 1 2024 (c) 2024 the authors2024 https://creativecommons org/licenses/by/4 0/ this is an open-access article distributed under the terms of the creative commons attribution license.; the assemblies are provided via figshare ( https://doi org/10 25392/leicester data 21346935 v2 )[ ]. data availability - all reads from virome sequencing were submitted to the ena under study prjeb56639"

Code Sharing
Evidence found in paper:

"Conflicts of interest The authors declare that there are no conflicts of interest."

Evidence found in paper:

"Funding information R.C. is supported by a scholarship from the Medical Research Foundation National PhD Training Programme in Antimicrobial Resistance Research (MRF-145–0004-TPG-AVISO). Bioinformatics analysis was carried out on infrastructure provided by MRC-CLIMB (MR/L015080/1)."

Protocol Registration
Open Access
Paper is freely available to read
Additional Indicators
Replication
Novelty Statement
Assessment Info

Tool: rtransparent

OST Version: N/A

Last Updated: Aug 05, 2025