Transcriptome Analysis Provides Insights into Catalpol Biosynthesis in the Medicinal Plant <i>Rehmannia glutinosa</i> and the Functional Characterization of <i>RgGES</i> Genes.

"the raw data from the transcriptomes were submitted to the publicly accessible genome sequence archive (gsa) database in the big data center under the accession number cra005581.; supplementary materials the following supporting information can be downloaded at https://www mdpi com/article/10 3390/genes15020155/s1 supplementary figure s1: the r glutinosa plant; supplementary figure s2: the flowchart to the material and method section for summarizing methodology; supplementary figure s3: the error rate distribution of unigenes; supplementary figure s4: the melting curves of the genes chosen for qrt-pcr analysis; supplementary figure s5: the heatmap of differentially expressed genes that were evaluated in this study; supplementary figure s6: agarose gel electrophoresis for pcr products of rgges ; supplementary figure s7: the sds-page analysis of the recombinant rgges1 and rgges2 protein; supplementary table s1: primers used in this study; supplementary table s2: transcriptome assembly statistics for r glutinosa ; supplementary table s3: annotation of unigenes in kog database; supplementary table s4: kegg classification of all the unigenes; supplementary table s5: summary of annotated unigenes in go database; supplementary table s6: candidate unigenes of common enzymes in route i and route ii ; supplementary table s7: candidate ugt unigenes in r glutinosa transcriptomes; supplementary table s8: accession numbers of pspgs used for phylogenetic analysis in figure 6 ; supplementary table s9: aldehyde dehydrogenase unigenes identified in r glutinosa transcriptomes; supplementary table s10: nadp + oxidoreductase candidate genes in r glutinosa transcriptomes; supplementary table s11: cyp450 unigenes identified in r glutinosa transcriptomes; supplementary table s12: putative dehydratase genes in r glutinosa transcriptomes; supplementary table s13: decarboxylase candidate genes in r glutinosa transcriptomes; supplementary table s14: putative hydroxylase and epoxidase genes involved in catalpol biosynthesis; supplementary table s15: accession numbers of tpss used for phylogenetic analysis in figure 8 .; data availability statement the transcriptome data have been deposited in the publicly accessible genome sequence archive (gsa) database in the big data center under the accession number cra005581. supplementary table s2 ). the raw data"

"Conflicts of Interest The authors declare no conflicts of interest."

"This research was funded by the National Natural Science Foundation of China, grant number 82204571."