Identification of a novel CG307 sub-clade in third-generation-cephalosporin-resistant <i>Klebsiella pneumoniae</i> causing invasive infections in the USA.

"collectively this study underscores the emergence and dissemination of a distinct cg307 sub-clade that is a prevalent cause of 3gc-r k pneumoniae bacteraemia among cancer patients seen in houston tx and has recently been isolated throughout the usa accessory genome clonal group 307 multidrug-resistant k pneumoniae surveillance nosocomial transmission third-generation-cephalosporin-resistant klebsiella pneumoniae openaccessembargo 0 whole-genome-sequencing (wgs) data collected during this study period was submitted to the national center for biotechnology information (ncbi) and can be accessed within bioproject prjna648389.; wgs data from a previous study of carbapenem non-susceptible enterobacterales can be accessed from bioproject prjna836696.; wgs workflow cg distributions and trends of 3gc-r k pneumoniae isolates ( a ) workflow of wgs inclusion/exclusion criteria for k pneumoniae bacteraemia isolates * 27 isolates from a previous study (bioproject accession no prjna836696) were included in our analyses ( b ) pie chart showing the cg distribution of index 3gc-r k pneumoniae isolates ( c ) cgs of index 3gc-r k pneumoniae isolates stratified by year."

"for cg307 population structure analysis we performed a convenience sampling of publicly available cg307 isolates from diverse geographical locales and collection dates specifically oversampling for cg307 isolates harbouring bla shv-205 alleles cg307 short-reads were downloaded from ncbi using the sratoolkit v2 10 9 through the fasterq-dump function ( https://github com/ncbi/sra-tools )."

"Conflicts of interest The authors declare that there are no conflicts of interest."

"Funding information Core grant CA016672 (Advanced Technology Genomics Core - ATGC) and NIH grant 1S10OD024977-01 provided funding for the ATGC sequencing facility at MDACC. S.S.A. was supported by a Dell Family Fund for the School of Health professional scholarship, and C.-T.W. was supported by a Peter and Cynthia Hu scholarship. W.C.S. was supported through the National Institute of Allergy and Infectious Diseases (NIAID) T32 AI141349 Training Program in Antimicrobial Resistance. Support for this study was also provided by NIAID grants R21AI151536 and P01AI152999 for S.A.S."